Background:

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L12, also known as RPL12, is a 165 amino acid protein that localizes to the cytoplasm and exists as a component of the 60S subunit through which it binds directly to 26S rRNA. Multiple isoforms of Ribosomal Protein L12 exist due to alternative splicing events. Like most ribosomal proteins, Ribosomal Protein L12 exists as multiple processed pseudogenes that are scattered throughout the genome

Specificity:

RPL12 (N103) polyclonal antibody detects endogenous levels of Ribosomal Protein L12 protein.

Alternative names:

60S ribosomal protein L12

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 72-121of Human RPL12.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 18 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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Email: info@sobekbio.com

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