Background:

Lambda 5 (also called immunoglobulin lambda-like polypeptide 1 or CD179b antigen) and VpreB comprise the surrogate light chain of the pre-B cell receptor complex. SL chain is also part of a quality control mechanism that tests a μ-chain for its ability to pair with conventional L chains. It can form Ig-like complexes with the heavy (H) chain, the DHJHC μ-protein or the p55 chain. Production of the surrogate light chain begins at the stage of pro-B cells, continues during the pre-B cell stage and halts at the immature B cell stage. Once pre-BCR is expressed, SL chain expression is turned off. As pre-B II cells proliferate, SL is diluted out, thus limiting pre-BCR formation. Lambda 5, a 22 kDa protein, is critical for B cell development in mammals. Expression of Lambda 5 is highest in liver, pre-B lymphocytes and bone marrow, the major source of B cell precursors.

Specificity:

CD179b (V26) polyclonal antibody detects endogenous levels of CD179b protein.

Alternative names:

Immunoglobulin lambda-like polypeptide 1; CD179 antigen-like family member B; Ig lambda-5; Immunoglobulin omega polypeptide; Immunoglobulin-related protein 14.1; CD179b; IGL1; IGLL1

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to the N-terminus of Human CD179b.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 25 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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