Background:

GnRH is produced by hypothalamic neurons and secreted in a pulsatile manner into the capillary plexus of the median eminence. GnRH affects the release of lutenizing hormone and follicle stimulating hormone from gonadotropic cells in the anterior pituitary. In addition to hypothalamic GnRH (GnRH I), a second GnRH form (GnRH II) functions primarily in the midbrain. GnRH is xpressed in the acrosomal region of human sperm and in the anterior pituitary tissue and cancer cells. Unlike GnRH I, GnRH II is highly expressed outside the brain, particularly in the kidney, bone marrow and prostate, suggesting that it may have multiple functions. GnRH binds to a specific G protein-coupled receptor in the pituitary to regulate synthesis and secretion of gonadotropins.

Specificity:

GnRH I (D43) polyclonal antibody detects endogenous levels of GnRH I protein.

Alternative names:

Progonadoliberin-1; Progonadoliberin I; Gonadoliberin-1; Gonadoliberin I; Gonadorelin; Gonadotropin-releasing hormone I; GnRH-I; Luliberin I; Luteinizing hormone-releasing hormone I; LH-RH I; GnRH-associated peptide 1; GnRH-associated peptide I; GNRH1; GNRH; GRH; LHRH

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 11-60 of Human GnRH I.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 11 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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