Background:

Golgin 45, also known as JEM-1 or basic leucine-zipper nuclear factor 1, is a 400 amino acid protein that localizes to the lumen of the Golgi apparatus. The primary function of golgin 45 is to maintain normal Golgi structure and to facilitate protein transport from the endoplasmic reticulum to the cell surface. The latter process is regulated primarily through the interaction of golgin 45 and GRASP55, which together form a Rab 2 effector complex. Excluding formation of a Rab 2 complex, golgin 45 does not interact with any other Golgi Rab proteins. Golgin 45 is expressed as two isoforms, one of which localizes to the nucleus and is thought to function as a transcription factor.

Specificity:

Golgin 45 (D22) polyclonal antibody detects endogenous levels of Golgin 45 protein.

Alternative names:

Golgin-45; Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor; BLZF1; JEM1

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to the N-terminual of Human Golgin 45.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 45 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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