Background:

FLIP (FLICE inhibitory protein) is expressed as both long and short forms and is involved in the regulation of apoptosis. The short form of FLIP contains two death effector domains homologous to the death effector domain of the Fas-associating protein FADD. The long form of FLIP, which shares significant homology with the cysteine protease FLICE, contains an additional caspase-like domain, but lacks a catalytic active site and lacks the residues that form the substrate binding pocket in most caspases. FLIP has been designated by independent groups as Casper, I-FLICE, CLARP, FLAME-1 and MRIT. Although its exact role is still being elucidated, FLIP appears to be an important factor in the regulation of apoptosis downstream of all known death receptors.

Specificity:

FLIP (H204) polyclonal antibody detects endogenous levels of FLIP protein.

Alternative names:

CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; Usurpin; CFLAR; CASH; CASP8AP1; CLARP; MRIT

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 171-200 of Human FLIP.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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