Background:

Pitx1 and Pitx2 are highly homologous, bicoid-related transcription factors. Pitx1 is a bicoid-related homeodomain factor that exhibits preferential expression in the hindlimb, as well as expression in the developing anterior pituitary gland and first branchial arch. Deletion of the Pitx1 locus results in decreased distal expression of the hindlimb-specific marker, the T-box factor (Tbx4). Pitx1 may modulate morphogenesis, growth and patterning of a specific hindlimb region, and serves as a component of the variables that influence morphological and growth distinctions in forelimb and hindlimb identity.

Specificity:

Pitx1 (E130) polyclonal antibody detects endogenous levels of Pitx1 protein.

Alternative names:

Hindlimb-expressed homeobox protein backfoot; PITX1; BFT. POTX. PTX1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 100-150 of Human Pitx1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 34 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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