Background:

This study investigates the involvement of α2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that α2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal α2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, α1D-AR, were not changed by the treatment. Moreover, αo-subunits of GTP-binding proteins (Gαo), one of the downstream molecules of α2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of α2A-AR in the mouse hippocampus.

Specificity:

AR α2A (R361) polyclonal antibody detects endogenous levels of AR α2A protein.

Alternative names:

Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR; ADRA2A; ADRA2R; ADRAR; α-2A adrenoceptor; α-2AAR; ARα-2A; AR α2A; ARα2A; ARα 2A;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 331-380 of Human AR-α2A.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 48, 54 kDa

Applications:

IHC IF

Usage:

IHC: 1:50~1:200 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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