Background:

Tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-α, also known as TNF−β) are members of a family of secreted and cell surface cytokines that participate in the regulation of immune and inflammatory responses. LT-β (lymphotaxin-beta or tumor necrosis factor C) is a type II membrane protein with significant homology to TNF, LT-α, and the ligand for the CD40 receptor. LT-α is present on the surface of activated T, B, and LAK cells as a complex with the 33 kda glycoprotein, LT-β. LT-β, also expressed by active lymphocytes, forms a heterotrimer with LT-a on the cell surface and anchors LT-α to the cell surface. A TNF receptor-related protein, the LT-β receptor (also known as TNFC receptor), is the human receptor for the LT-α/LT-β heterotrimer. There are two LT-β isoforms expressed in human lymphoid cell lines and non-Hodgkin's lymphomas. The gene which encodes LT-β maps to the major histocompatibility complex region on human chromosome 6p21.3.

Specificity:

LT-β (V211) polyclonal antibody detects endogenous levels of LT-β protein.

Alternative names:

Lymphotoxin-beta; LT-beta; Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3; LTB; TNFC; TNFSF3

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 200-250 of Human LT-β.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 25 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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