Background:

The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3,but not Sp2 or Sp4, interact with E2, a regulatory element for the β4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription.

Specificity:

Sp3/4 (G659) polyclonal antibody detects endogenous levels of Sp3 and Sp4 protein.

Alternative names:

Transcription factor Sp3; SPR-2; SPR2; SP3; Transcription factor Sp4; SPR-1; SPR1; SP4

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 630-680 of Human Sp3.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 85 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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