Background:

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of β-adrenergic receptor kinase, GRK 2 (βARK, βARK1) and GRK 3 (βARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Specificity:

GRK 2 (D635) polyclonal antibody detects endogenous levels of GRK 2 protein.

Alternative names:

Beta-adrenergic receptor kinase 1; Beta-ARK-1; β-ARK-1; ARK-β1; G-protein coupled receptor kinase 2; ADRBK1; BARK; BARK1; GRK2; ARK β1; ARKβ1; β-ARK1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 600-650 of Human GRK2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 80 kDa

Applications:

WB IHC IP

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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