Background:

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis. There are three isozymes: muscle-type, liver-type, and platelet-type. The liver-type PFK (PFKL) is the predominant subunit of PFK found in the liver. Research studies have shown that because of its location on chromosome 21, this enzyme is overexpressed in Down Syndrome/trisomy 21. PFKL also may have a role in the physiological response to a low fat diet.

Specificity:

PFKL (E724) polyclonal antibody detects endogenous levels of PFKL protein.

Alternative names:

6-phosphofructokinase; liver type; Phosphofructokinase 1; Phosphohexokinase; Phosphofructo-1-kinase isozyme B; PFK-B;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 700-750 of Human PFKL.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 85 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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