Background:

AKAP 10 (A kinase anchor protein 10), also known as PRKA10 or D-AKAP2 (dual-specific A kinase-anchoring protein 2), is a 662 amino acid mitochondrial membrane protein that belongs to the AKAP family. AKAP 10 is a dual specificity protein that binds to both type I and type II regulatory subunits of PKA and anchors them to the plasma membrane or the mitochondria. When anchored to the mitochondria, PKA can phosphorylate and, thus, inactivate the proapoptotic protein Bad. This suggests that AKAP 10 indirectly regulates Bad-induced apoptosis by mediating the mitochondrial attachment of PKA. Additionally, AKAP 10 may facilitate G-protein coupled signal transduction and could act as an adaptor in the assembly of multiprotein complexes.

Specificity:

AKAP 10 (R27) polyclonal antibody detects endogenous levels of AKAP 10 protein.

Alternative names:

A-kinase anchor protein 10, mitochondrial; AKAP-10; Dual specificity A kinase-anchoring protein 2; D-AKAP-2; Protein kinase A-anchoring protein 10; PRKA10; AKAP10

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 1-50 of Human AKAP 10.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 74 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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