Background:

Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1α and IL-1β, and a specific receptor antagonist, IL-1Rα. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFκB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB.

Specificity:

IRAK1 (T381) polyclonal antibody detects endogenous levels of IRAK1 protein.

Alternative names:

Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1; IRAK

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 350-400 of Human IRAK1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 76 kDa

Applications:

WB IF

Usage:

WB: 1:500~1:1000 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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