Background:

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor uperfamily of ligand-activated transcription factors. Estrogen receptors, ncluding ERα and ERβ, contain DNA binding and ligand binding domains nd are critically involved in regulating the normal function of reproductive issues. ERα and ERβ have been shown to be differentially activated by various igands. Receptor-ligand interactions trigger a cascade of events, including issociation from heat shock proteins, receptor dimerization, phosphorylation nd the association of the hormone activated receptor with specific regulatory lements in target genes. Evidence suggests that ERα and ERβ may be egulated by distinct mechanisms even though they share many functional characteristics.

Specificity:

ER-α (K531) polyclonal antibody detects endogenous levels of ER-α protein.

Alternative names:

Estrogen receptor; ER-alpha; ER; ESR1; ESR; NR3A1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 501-550 of Human ERα.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 66 kDa

Applications:

IHC IF

Usage:

IHC: 1:50~1:200 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com