Background:

TUB4, the gene for Saccharomyces cerevisiae γ-Tubulin, encodes a 473 amino acid structural protein that localizes to the spindle pole body. γ Tubulin, an essential protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. γ Tubulin-depleted cells fail to form functional spindles and arrest during nuclear division. γ Tubulin associates with spindle body components Spc97 and Spc98 to form the γ Tubulin complex. The budding yeast γ Tubulin complex contains one molecule each of Spc97 and Spc98 and two molecules of γ Tubulin. In the SPB, Spc110 binds Spc97 and Spc98 of the γ Tubulin complex. 2D gel analysis indicates five isoforms of γ Tubulin. The phosphorylation of γ Tubulin at Tyr 445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1.

Specificity:

Tubulin γ (F414) polyclonal antibody detects endogenous levels of Tubulin γ protein.

Alternative names:

Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1; TUBG1; TUBG; Tubulin γ1; Tubulin-γ1; Tubulinγ1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 380-430 of Human Tubulin γ.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 51 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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