Background:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

Specificity:

Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein.

Alternative names:

Total tubulin β; Tubulin beta

Species reactivity:

Human,Mouse, Rabbit, Frog, Fish, Chicken, Rat

Host:

Mouse

Ig type:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β.

Purification & Purity:

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

WB IHC IF

Usage:

WB: 1:5000~20000 IHC: 1:100~500 IF: 1:100~500

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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