Background:

NEDD8 is a ubiquitin-like protein that controls vital biological events through its conjugation to cullin proteins. NEDD8 ultimate buster-1 (NUB1), is a negative regulator of the NEDD8 system that recruits NEDD8 and its conjugates to the proteasome for degradation. It is, therefore a cell growth regulator. The UBA domain of NUB1 is the specific acceptor for the linear ubiquitin precursor. NUB1 is composed of 601 amino acids. It is an interferon-inducible protein and predominantly localizes in the nucleus. NUB1 is specifically expressed in adult human testis, ovary, heart, and skeletal muscle tissues.

Specificity:

NUB1 (Y601) polyclonal antibody detects endogenous levels of NUB1 protein.

Alternative names:

NEDD8 ultimate buster 1; Negative regulator of ubiquitin-like proteins 1; Renal carcinoma antigen NY-REN-18; NUB1; NYREN18

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 570-621 of Human NUB1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 70 kDa

Applications:

WB IHC IP

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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