Background:

Transforming growth factor betas (TGFβs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic develop ment. Three TGFβs have been identified in mam mals. TGFβ1, TGFβ2 and TGFβ3 are each syn thesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the mol ecules. Biologically active TGFβ requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFβ3 protein has approx imately 80% identity to the mature region of both TGFβ1 and TGFβ2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

Specificity:

TGF β3 (G292) polyclonal antibody detects endogenous levels of pro-TGFβ3 (47 kDa) and Cleaved-TGFβ3 (13 kDa) protein.

Alternative names:

Transforming growth factor beta-3; TGF-beta-3; Latency-associated peptide; LAP; TGFB3; TGFβ3; TGF-β3

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 261-310 of Human TGF β3.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 50 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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