Background:

Transforming growth factor α (TGFα) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFα is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFα binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor β. TGFα is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFα bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Specificity:

TGF α (R139) polyclonal antibody detects endogenous levels of TGF α protein.

Alternative names:

Protransforming growth factor alpha; Transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1; TGFA; TGF α

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 111-160 of Human TGFα.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 10, 17 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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