Background:

The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.

Specificity:

PML (P39) polyclonal antibody detects endogenous levels of PML protein.

Alternative names:

Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19; MYL; PP8675; RNF71; TRIM19

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to the N-terminal of Human PML.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 98 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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