Background:

Dynactin is a multisubunit complex that functions as a binding partner for the Dynein microtubule motor. Dynactin-Dynein binding may be required for most, if not all, cytoplasmic Dynein-driven activities and is thought to contribute to the functional diversity of Dynein. Dynactin 2, also known as DCTN2, Dynamitin or DCTN50, is a peripheral membrane protein that is one of many subunits in the Dynactin complex. Like other Dynactin subunits, Dynactin 2 mediates Dynein-organelle binding and helps to regulate chromosome alignment during prometaphase and spindle organization during mitosis. Overexpression of Dynactin 2 disrupts the Dynactin-Dynein complex, thus inhibiting retrograde axonal transport and causing motor neuron degeneration. Additionally, overexpression of Dynactin 2 may disrupt the cell cycle and lead to osteosarcoma, suggesting a possible role for Dynactin 2 in carcinogenesis

Specificity:

Dynactin 2 (L371) polyclonal antibody detects endogenous levels of pDynactin 2 protein.

Alternative names:

Dynactin subunit 2; 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitin; DCTN2; DCTN50

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 340-390 of Human Dynactin 2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 44 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com