Background:

Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific α subunit (IL-3Rα) and a signal transducing β subunit (IL-3Rβ) common to the IL-3/IL-5/GM-CSF receptors. The α subunits are low-affinity ligand-binding proteins while the β subunits do not themselves bind ligand, but are required for high affinity binding by the α subunits. The mouse IL-3 receptor has two distinct β subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine β subunits are 91% homologous at the amino acid level but only 56% homologous to the human β subunit.

Specificity:

IL-3R (F587) polyclonal antibody detects endogenous levels of IL-3R protein.

Alternative names:

Cytokine receptor common subunit beta; CDw131; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131; CSF2RB; IL3RB; IL5RB; IL3Rβ

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 550-600 of Human IL-3Rβ.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 130 kDa

Applications:

IHC

Usage:

IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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