Background:

ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes.

Specificity:

ULK1 (phospho-Ser555) polyclonal antibody detects endogenous levels of ULK1 protein only when phosphorylated at Ser555.

Alternative names:

Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; Unc-51-like kinase 1; ULK1; KIAA0722

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phosphopeptide derived from human ULK1 around the phosphorylation site of Ser555.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 150 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com