Background:

RASA4 (ras GTPase-activating protein 4) is an 803 amino acid member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogenactivated protein kinase pathway in response to Ca2+. Stimuli that increase intracellular Ca2+ levels result in the translocation of the RASA4 protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth and differentiation. Containing 21 exons and spanning 35 kb of genomic DNA, RASA4 includes one Btk-type zinc finger, two C2 domains, one PH domain and one Ras-GAP domain.

Specificity:

RASA4 polyclonal antibody detects endogenous levels of RASA4 protein.

Alternative names:

Ras GTPase-activating protein 4; Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2; CAPRI; GAPL; KIAA0538

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

A synthetic peptide corresponding to residues in Human RASA4.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 85, 90 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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