Background:

MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway. MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α. Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

Specificity:

MEK-7 (phospho-S271) polyclonal antibody detects endogenous levels of MEK-7 protein only when phosphorylated at Ser271.

Alternative names:

Mitogen Activated Protein Kinase kinase 7; c-Jun N-terminal kinase kinase 2; JNK activating kinase 2; Jnkk 2; Jnkk2; MAP2K7; MAPKK-7; MKK 7; MKK-7; PRKMK 7; PRKMK7; Sek-2; Dual specificity mitogen activated protein kinase kinase 7; JNK kinase 2; Jnkk

Species reactivity:

Human,Rat,Mouse

Host:

Rabbit

Ig type:

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Immunogen:

Synthetic phosphopeptide derived from human MEK-7 around the phosphorylation site of Serine 271

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 48 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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