Background:

In eukaryotes, ribosomal RNA genes are transcribed by RNA polymerase (pol I). In Saccharomyces cerevisiae, transcription of rRNA genes requires at least three transcription factors, which include the two multisubunit factors Core factor and UAF that function in the assembly of the preinitiation complex. The third factor, Rrn3, functions as a single subunit and is not required for the preinitiation complex assembly. Unlike other pol I transcription factors, Rrn3 is functionally conserved between yeast and mammals as an rRNA gene transcription regulator. Human Rrn3 is 21% homologous to the yeast Rrn3 protein and is a member of a conserved gene family spanning the fungi, plant and animal kingdoms. hRrn3 is highly expressed in the lung, retina, thymus, and prostate. Rrn3 may be identical to the transcription factor TIF-IA, since both TIF-IA and Rrn3 associate with pol I and their activities are growth rate dependent.

Specificity:

RRN3 (phospho-S649) polyclonal antibody detects endogenous levels of RRN3 protein only when phosphorylated at Ser649 .

Alternative names:

RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA; RRN3; TIFIA

Species reactivity:

Human

Host:

Rabbit

Ig type:

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Immunogen:

Synthetic phosphopeptide derived from human RRN3 around the phosphorylation site of Serine649.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 80 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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