Background:

TRIP12 (thyroid hormone receptor interactor 12) is a 1,992 amino acid E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway. TRIP12 also modulates the NEDD8 pathway, a series of steps implicated in signal transduction and cell cycle progression, where it influences APPBP1 degradation by catalyzing its ubiquitination. A member of the UPL family and K-HECT subfamily, TRIP 12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site. Subject to post-translational phosphorylation upon DNA damage, TRIP12 expression is highest in testis and skeletal muscle, and has also been found in heart, spleen, thymus, ovary, placenta, kidney, prostate and peripheral blood leukocytes at lower levels.

Specificity:

TRIP12 polyclonal antibody detects endogenous levels of TRIP12 protein.

Alternative names:

E3 ubiquitin-protein ligase TRIP12; E3 ubiquitin-protein ligase for Arf; Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12; TRIP12; KIAA0045; ULF

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human TRIP12.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 260 kDa

Applications:

WB IHC IF

Usage:

WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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