Background:

Secreted leucine-rich repeat-containing proteins 1 through 3 (Slit1-3) are secreted glycoproteins that influence axonal guidance and mediate normal neural progression by acting as high-affinity signaling ligands for the repulsive guidance receptors, robo1 and robo2 (also designated roundabout 1 and 2). Interactions between the robo receptor and Slit ligand families of proteins initiate signaling cascades that repel axonal outgrowth. The arrangement of the extracellular domains of robo4 diverges significantly from that of all other robo family members. Robo4 is the only robo family member expressed in primary endothelial cells. It binds Slit and inhibits cellular migration in a heterologous expression system. Together, the robo proteins prescribe developmental paths during neural development.

Specificity:

ROBO4 polyclonal antibody detects endogenous levels of ROBO4 protein.

Alternative names:

Roundabout homolog 4; Magic roundabout

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human ROBO4.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 130 kDa

Applications:

WB

Usage:

WB 1:200 - 1:500

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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