Background:

NUDC (nuclear distribution gene C homolog, A. nidulans) is a ubiquitously expressed protein that is conserved from fungus to human. Highly expressed in proliferating cells, NUDC localizes to the cytoplasm and nucleus, contains a CS domain and participates in neurogenesis, mitosis, nuclear migration and cytokinesis. At the onset of mitosis, NUDC is phosphorylated by Plk. This modification of NUDC is required for proper mitotic spindle formation, chromosome separation during mitosis, cytokinesis and cell proliferation. In neurons and fibroblasts, NUDC forms a complex with LIS1 that localizes to the microtubule network and microtubule-organizing center and facilitates nuclear movement and transport in migrating neurons. In addition, the NUDC/LIS1 complex can associate with the minus-end directed Dynein/Dynactin motor complex and, together, these complexes cooperate in the regulation of cytokinesis. NUDCD3 is a 361 amino acid protein that contains one CS domain and is phosphorylated upon DNA damage by either ATR or ATM.

Specificity:

NUDC polyclonal antibody detects endogenous levels of NUDC protein.

Alternative names:

Nuclear migration protein nudC; Nuclear distribution protein C homolog; NUDC

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human NUDC.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 45 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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