Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX1 mRNA has a widespread distribution in human fetal tissue, but is not uniformly expressed in all tissues. Chicken DDX1, which shares 93% identity with human DDX1, shows highest levels of expression during the early stages of development. Tissue maturation typically correlates with a decrease in DDX1 expression, although DDX1 levels remain elevated in late embryonic retina and brain.

Specificity:

DDX1 polyclonal antibody detects endogenous levels of DDX1 protein.

Alternative names:

ATP-dependent RNA helicase DDX1; DEAD box protein 1; DEAD box protein retinoblastoma; DBP-RB; DDX1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human DDX1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 100 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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