Background:

Interphotoreceptor retinoid-binding protein (IRBP) is a retinoid and fatty acid binding protein expressed exclusively in the photoreceptor cells of the retina and pinealocytes of the pineal gland. The gene encoding human IRBP maps to chromosome 10q11.2. A putative trans-acting complex binds a cis-element in the IRBP promoter and fully activates transcription of the IRBP gene. Hypomethylation of cysteine residues in the IRBP promoters of bovine and murine photoreceptor cells permits expression of the IRBP gene. IRBP may be involved in shuttling retinoids between photoreceptors and the retinal pigment epithelium. IRBP exists as a glycoprotein with a four-fold repeat structure. Each 300 amino acid repeat contains one A and one B domain corresponding to the first 80 amino acids (A) and the other 220 amino acids (B). Along with S-antigen and opsin, the antigenic properties of IRBP induce posterior uveitis, which is characterized by the inflammation of the choroid and photoreceptor cell death.

Specificity:

RBP3 polyclonal antibody detects endogenous levels of RBP3 protein.

Alternative names:

Retinol-binding protein 3; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein; RBP3

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RBP3.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 180 kDa

Applications:

WB IHC IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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