Background:

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

Specificity:

SF3B2 polyclonal antibody detects endogenous levels of SF3B2 protein.

Alternative names:

Splicing factor 3B subunit 2; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SF3b145; SF3b150; Spliceosome-associated protein 145; SAP 145; SF3B2; SAP145

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human SF3B2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 140 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com