Background:

Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells. The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis. In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology. NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis. NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy.

Specificity:

NRF-1 polyclonal antibody detects endogenous levels of NRF-1 protein.

Alternative names:

Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human NRF-1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 68 kDa

Applications:

WB IF

Usage:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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