CUL4B polyclonal antibody BS7768
Specifications
| 50ul/100ul |
Background:
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. Cul-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/Cul-1/F-box protein complex) consists of Skp1 associating with both Cul-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. Cul-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
Specificity:
CUL4B polyclonal antibody detects endogenous levels of CUL4B protein.
Alternative names:
CUL 4B; CUL-4B; CUL4B; CUL4B_HUMAN; Cullin-4B; DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2;
Species reactivity:
Human,Mouse,Rat
Host:
Rabbit
Ig type:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen:
Recombinant full length Human CUL4B.
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Molecular weight:
~ 115 kDa
Applications:
WB IHC IF
Usage:
WB: 1:500~1:2000 IHC/IF: 1:50~1:200
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Precautions:
For research use only, not for use in diagnostic procedure.
More info:
Email: info@sobekbio.com
Orders:
Email: orders@sobekbio.com