Background:

In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. The eukaryotic translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex. eIF5B (Eukaryotic translation initiation factor 5B), also known as Translation initiation factor IF-2, is a 1,120 amino acid cytoplasmic protein that functions in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. eIF5B interacts with Annexin V, an anticoagulant protein, in a calcium and phospholipid-dependent manner. Since eIF5B is conserved among all three kingdoms of life, it is also known as an universal initiation factor.

Specificity:

EIF5B polyclonal antibody detects endogenous levels of EIF5B protein.

Alternative names:

Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2; EIF5B; IF2; KIAA0741

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human EIF5B.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 179 kDa

Applications:

WB,IF

Usage:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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