Background:

EMSY interacts with BRCA2 and plays a role in chromatin remodeling. This interaction has been confirmed in HeLa cells. Overexpression of EMSY strongly correlates with amplification in sporadic breast cancer and higher grade ovarian cancer. The EMSY gene is amplified in 18% of breast cancer cell lines. EMSY amplification is highly correlated with DNA amplification in both cell lines and primary tumors. This amplification is a general sign of poor prognosis and shortened disease-free survival time. EMSY from a wide variety of species has a conserved 80 amino acid sequence at the N-terminus. In irradiated MEFs (mouse embryonic fibroblasts), EMSY was found to migrate to damaged DNA.

Specificity:

C11orf30 polyclonal antibody detects endogenous levels of C11orf30 protein.

Alternative names:

C11orf30; Chromosome 11 open reading frame 30; EMSY protein; FLJ90741; GL002; Protein EMSY;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human C11orf30.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

Refer to figures

Applications:

WB IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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