Background:

Mammalian Toll-like receptors (TLRs) recognize conserved products of microbial metabolism and activate NF˚B and other signaling pathways through the adapter protein MyD88. MyD88 functions as an adapter protein in the association of IL-1 receptor associated kinase (IRAK) with the IL-1 receptor. MyD88 contains a characteristic N-terminal death domain, which is essential for NF˚B activation, and an adjacent Toll/Il-1R homology domain (TIR domain), which is responsible for signal transduction. TIR domain-containing adapter protein (TIRAP), also designated MAL (MyD88 adapter-like), wyatt or TLR-4 adaptor protein, is a cytoplasmic TIR-domain-containing protein that activates NF˚B, Jun N-terminal kinase and extracellular signal-regulated kinase-1 and -2. TIRAP forms homodimers and heterodimers with MyD88. IRAK-2, but not IRAK, is required for the activation of NF˚B by TIRAP which associates with IRAK-2 through the TIR domain. In addition, TIRAP associates with TLR-4, suggesting that it plays a role in TLR-4 signal transduction.

Specificity:

TIRAP polyclonal antibody detects endogenous levels of TIRAP protein.

Alternative names:

Adapter protein wyatt; FLJ42305; MAL; MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human TIRAP.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 32 kDa

Applications:

WB IHC IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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