Background:

Acrogranin (also designated PC cell-derived growth factor (PCDGF), epithelin/granulin precursor or paragranulin) is a glycosylated protein originally purified from the highly tumorigenic, Insulin-independent mouse teratoma PC cell line. Acrogranin is a cysteine-rich molecule whose expression is essential for tumorigenicity in teratoma cells. Acrogranin is expressed in estrogen receptor-positive (ER(+)) human mammary MDA-MB-468 epithelial cells, human breast cancer MCF7 cells and human estrogen-responsive T-47D cells. Secreted acrogranin acts as an autocrine growth factor for breast carcinoma cells and overexpression may play an important role in human breast cancer. Acrogranin stimulates the growth of PC cells as well as 3T3 fibroblasts.

Specificity:

GRN polyclonal antibody detects endogenous levels of GRN protein.

Alternative names:

Acrogranin; CLN11; GEP; GP88; Granulin A; Granulin B; Granulin C; Granulin D; Granulin E; Granulin epithelin; Granulin F; Granulin G; Granulin-7; Granulins; GRN; GRN_HUMAN; PC cell derived growth factor; PCDGF; PEPI; PGRN; Proepithelin; Progranulin;

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human GRN.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 80 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com