Background:

NEDD4 is a 221 amino acid, Golgi-associated protein which contains three transmembrane domains in its carboxy-terminus and two PY motifs in its amino-terminus. NEDD4 may play a role in Golgi structure and function. It binds the WW domains of a number of NEDD4 family members called HECT-type E3 ubiquitin ligases. NEDD4 is strongly expressed in surviving neurons around an injury site, which suggests that ubiquitination may be a possible survival strategy and that NEDD4 may act as a neuroprotective protein. It may also have an effect on the function of Itch, another E3 ubiquitin ligase. Expression of NEDD4 and its association with Itch may be promoted by T cell activation.

Specificity:

NEDD4 polyclonal antibody detects endogenous levels of NEDD4 protein.

Alternative names:

NEDD-4; NEDD4; KIAA0093; NEDD4-1; PIG53

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human NEDD4.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 120 kDa

Applications:

WB IHC

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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