Background:

Hemoglobin (Hgb) is coupled to four iron-binding,methene-linked tetrapyrrole rings(heme). The α (16p13.3; 5'-ζ-pseudoζ-pseudoα2-pseudoα1-α2-α1-θ1-3') and β (11p15.4) globin loci determine the basic hemoglobin structure.The globin portion of Hgb consists of twoαchains and two β chains arranged in pairsforming a tetramer. Each of the four globin chainscovalently associates with a heme group. The bonds between α and β chains are weaker than between similar globin chains, therebyforming a cleavage plane that is important for oxygen binding and release. High affinityfor oxygen occurs upon relaxation of theα1-β2 cleavage plane. When the twoα1-β2 interfaces are closely bound, hemoglobin has a lowaffinityfor oxygen. Hb A,which containstwo α chains plustwo β chains,comprises 97% of totalcirculating hemoglobin. The remaining 3% of totalcirculating hemoglobin iscomprised of Hb A-2,which consists of twoαchains plustwo δ chains, and fetal hemoglobin (Hb F),which consists of two α chainstogetherwith two γ chains.

Specificity:

HBB polyclonal antibody detects endogenous levels of HBB protein.

Alternative names:

Hemoglobin subunit beta; Beta-globin; Hemoglobin beta chain; Cleaved into the following 2 chains:LVV-hemorphin-7; Spinorphin

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human HBB.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 16 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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