Background:

DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX8 (DEAH box polypeptide 8), also known as DHX8, HRH1 or PRP22, contains an arginine- and serine-rich domain (RS domain) that is characteristic of some splicing factors. DDX8 may be targeted to the spliceosome through an interaction involving its RS domain.

Specificity:

DHX8 polyclonal antibody detects endogenous levels of DHX8 protein.

Alternative names:

ATP-dependent RNA helicase DHX8;DEAH box protein 8;RNA helicase HRH1;DHX8;DDX8

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Immunogen:

Synthetic peptide, corresponding to Human DHX8 .

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 140kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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