Background:

p53 is a transcription factor that mediates cell cycle arrest and apoptosis by binding to DNA and activating the transcription of specific genes. p53 is also thought to be involved in DNA repair by the transcriptional activation of a ribonucleotide reductase gene, p53R2, after exposure to genotoxic stresses. p53R2 displays a significant similarity to ribonucleotide reductase small subunit (R2), and the expression of R2 is elevated at the onset of the S-phase of the cell cycle. However, only p53R2 expression is induced in response to ultraviolet and g-irradiation and adriamycin treatment. p53R2 translocates to the nucleus upon DNA damage, and subsequently, supplies an immediate pool of dNTPs necessary for DNA repair.

Specificity:

p53R2 (F156) polyclonal antibody detects endogenous levels of p53R2 protein.

Alternative names:

RRM 2B; RRM2B; p53R2; p53 R2; MGC42116; MTDPS8A; MTDPS8B;

Species reactivity:

Human,Rat,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to amino acids 123-178 of Human p53R2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 40 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

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