Background:

Serine/threonine-protein phosphatase 4 regulatory subunit 2, regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.

Specificity:

PPP4R2 polyclonal antibody detects endogenous levels of PPP4R2 protein.

Alternative names:

Serine/threonine-protein phosphatase 4 regulatory subunit 2; PPP4R2; SBBI57

Species reactivity:

Human,Rat,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Immunogen:

Synthetic peptide, corresponding to Human PPP4R2.

Purification & Purity:

The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

WB IHC

Usage:

WB: 1:500~1000

Storage:

Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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