Background:

The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP21A2 localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Activity of CYP21A2 is required for the synthesis of steroid hormones, including cortisol and aldosterone. Mutations in this gene is the primary cause of congenital adrenal hyperplasia (CAH), an autosomal recessive disorder. Gene conversion events involving the functional CYP21A2 gene (C21B) and a related pseudogene (C21A) located near the C21B gene may account for the majority of cases of steroid 21-hydroxylase deficiency.

Specificity:

Cytochrome P450 21A2 polyclonal antibody detects endogenous levels of Cytochrome P450 21A2 protein.

Alternative names:

Steroid 21-hydroxylase; 21-OHase; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 XXI; Cytochrome P450-C21; Cytochrome P450-C21B; CYP21A2; CYP21; CYP21B

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to Human Cytochrome P450 21A2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 50 kDa

Applications:

WB

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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