Background:

Mitochondrial translation is critical for maintaining function and preventing impairment of mitochondrial DNA, with mitochondrial translation elongation factors playing an essential role in this process. EF-G2 (elongation factor G2), also known as RRF2mt (ribosome-releasing factor 2, mitochondrial), GFM2 or MSTP027, is a 779 amino acid protein belonging to the GTP-binding elongation factor family and EF-G/EF-2 subfamily. Localizing to mitochondrion, EF-G2 is widely expressed with high levels found in adult heart, skeletal muscle and kidney as well as adult and fetal liver. EF-G2 mediates ribosome recycling and by interacting directly with MRRF on the ribosome large subunit, is required for ribosome dissociation from mRNA. Existing as five alternatively spliced isoforms, the gene encoding EF-G2 maps to human chromosome 5q13.3.

Specificity:

GFM2 polyclonal antibody detects endogenous levels of GFM2 protein.

Alternative names:

Ribosome-releasing factor 2, mitochondrial; RRF2mt; Elongation factor G 2, mitochondrial; EF-G2mt; mEF-G 2; Elongation factor G2; hEFG2; GFM2; EFG2; MSTP027

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to Human GFM2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 95 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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