Background:

Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. POLR3E, also known as DNA-directed RNA polymerase III subunit RPC5, is a 708 amino acid nuclear protein that is one of the 17 subunits that comprise Pol III. Specifically, POLR3E is a DNA-dependent RNA polymerase that uses the four ribonucleoside triphosphates to catalyze the transcription of DNA to RNA. The gene encoding POLR3K maps to human chromosome 16, which encodes over 900 genes in approximately 90 million base pairs and is associated with a variety of genetic disorders.

Specificity:

POLR3E polyclonal antibody detects endogenous levels of POLR3E protein.

Alternative names:

DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; DNA-directed RNA polymerase III 80 kDa polypeptide; POLR3E; KIAA1452

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic peptide, corresponding to Human POLR3E.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 73 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:1000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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