Background:

Crystallins are the major proteins expressed in the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into a, b and g families; b and g-crystallins compose a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. a-crystallins consist of three gene products, aA, aB and aC-crystallin, which are members of the small heat shock protein family (HSP20). They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, a-crystallins do not renature these proteins. Research indicates that binding occurs between membranes and aC-crystallin. The binding site appears to be at the polar-apolar interface in membrane protein (MIP26) and aC-crystallin; the lipid bilayer becomes less mobile with aC-crystallin binding.

Specificity:

HSPB8 polyclonal antibody detects endogenous levels of HSPB8 protein.

Alternative names:

Alpha crystallin C chain; Alpha-crystallin C chain; Charcot Marie Tooth disease axonal type 2L; Charcot Marie Tooth disease spinal; CMT2L; CRYAC; DHMN 2; DHMN2; E2 induced gene 1 protein; E2-induced gene 1 protein; E2IG1; H11; Heat shock 22kDa protein 8; Heat shock 27kDa protein 8; Heat shock protein 22; Heat shock protein beta 8; Heat shock protein beta-8; Hereditary motor neuropathy distal; HMN 2; HMN2; HMN2A; HSB8; HSPB 8; Hspb8; HSPB8_HUMAN; OTTHUMP00000239768; Protein kinase H11; Small stress protein like protein HSP22; Small stress protein-like protein HSP22; Spinal muscular atrophy distal adult autosomal dominant;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human HSPB8.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 22 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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