Background:

eukaryotic translation initiation factor 3 subunit D(EIF3D) Homo sapiens Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008],

Specificity:

eIF3ζ Polyclonal Antibody detects endogenous levels of eIF3ζ protein.

Alternative names:

EIF3D; EIF3S7; Eukaryotic translation initiation factor 3 subunit D; eIF3d; Eukaryotic translation initiation factor 3 subunit 7; eIF-3-zeta; eIF3 p66

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Immunogen:

The antiserum was produced against synthesized peptide derived from human EIF3D. AA range:101-150

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Molecular weight:

~ 64 kDa

Applications:

WB,IHC-p,IF(paraffin section),ELISA

Usage:

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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Email: info@sobekbio.com

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