Background:

hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7(HSD3B7) Homo sapiens This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008],

Specificity:

3β-HSD7 Polyclonal Antibody detects endogenous levels of 3β-HSD7 protein.

Alternative names:

HSD3B7; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase; C(27) 3-beta-HSD; Cholest-5-ene-3-beta; 7-alpha-diol 3-beta-dehydrogenase

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Immunogen:

The antiserum was produced against synthesized peptide derived from human HSD3B7. AA range:121-170

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Molecular weight:

~ 41 kDa

Applications:

WB,ELISA

Usage:

Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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