Background:

acetyl-CoA acetyltransferase 1(ACAT1) Homo sapiens This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009],

Specificity:

ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein.

Alternative names:

ACAT1; ACAT; MAT; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2

Species reactivity:

Human

Host:

Rabbit

Ig type:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Immunogen:

The antiserum was produced against synthesized peptide derived from human ACAT1. AA range:221-270

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Molecular weight:

~ 45 kDa

Applications:

WB,IHC-p,IF(paraffin section),ELISA

Usage:

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For

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